anti ctsk (Proteintech)
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Anti Ctsk, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ctsk/Cathepsin+K+Antibody/pmc13049295-26-3-5
Average 95 stars, based on 144 article reviews
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Immunohistochemistry:Article Title: EBV Promotes Alveolar Trabecula Resorption via Extracellular Vesicle Remodeling by Group IIA Secreted Phospholipase A2. Article Snippet: .. Immunohistochemistry and in situ hybridization Immunohistochemistry and EBER in situ hybridization were performed using a Leica Bond Max autostainer following the manufacturer’s instructions (Leica K.K., Tokyo, Japan) AntiCD20 (CD20-L26-L-CE, Novocastra, Wetzlar, Germany), In Situ Hybridization:Article Title: EBV Promotes Alveolar Trabecula Resorption via Extracellular Vesicle Remodeling by Group IIA Secreted Phospholipase A2. Article Snippet: .. Immunohistochemistry and in situ hybridization Immunohistochemistry and EBER in situ hybridization were performed using a Leica Bond Max autostainer following the manufacturer’s instructions (Leica K.K., Tokyo, Japan) AntiCD20 (CD20-L26-L-CE, Novocastra, Wetzlar, Germany), Article Title: EBV promotes alveolar trabecula resorption via extracellular vesicle remodeling by group IIA secreted phospholipase A 2 Article Snippet: Immunohistochemistry and EBER in situ hybridization were performed using a Leica Bond Max autostainer following the manufacturer’s instructions (Leica K.K.) .. Anti-CD20 (CD20-L26-L-CE, Novocastra), Membrane:Article Title: Single-cell analysis links DCUN1D5 to immune remodeling and cisplatin resistance in recurrent osteosarcoma. Article Snippet: The proteins are separated via 10% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Sigma-Aldrich, USA). .. The membrane is incubated with primary antibodies: anti-DCUN1D5 (1:1000, proteintech), anti-MMP9 (1:500, proteintech), Article Title: Single-cell analysis links DCUN1D5 to immune remodeling and cisplatin resistance in recurrent osteosarcoma Article Snippet: The proteins are separated via 10% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Sigma-Aldrich, USA). .. The membrane is incubated with primary antibodies: anti- DCUN1D5 (1:1000, proteintech), anti-MMP9 (1:500, proteintech), Incubation:Article Title: Single-cell analysis links DCUN1D5 to immune remodeling and cisplatin resistance in recurrent osteosarcoma. Article Snippet: The proteins are separated via 10% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Sigma-Aldrich, USA). .. The membrane is incubated with primary antibodies: anti-DCUN1D5 (1:1000, proteintech), anti-MMP9 (1:500, proteintech), Article Title: Single-cell analysis links DCUN1D5 to immune remodeling and cisplatin resistance in recurrent osteosarcoma Article Snippet: The proteins are separated via 10% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Sigma-Aldrich, USA). .. The membrane is incubated with primary antibodies: anti- DCUN1D5 (1:1000, proteintech), anti-MMP9 (1:500, proteintech), Article Title: Immune-Derived THBS1-CD47 Axis Induces Cellular Senescence and Suppresses Osteogenesis in Diabetic Periosteum Article Snippet: Subsequently, tissues were blocked by serum (Invitrogen) for 30 minutes at room temperature. .. Primary antibodies, including Western Blot:Article Title: Spinosin Suppresses RANKL-induced Osteoclastogenesis and Alleviates LPS-induced Cranial Osteolysis: A Study Based on Network Pharmacology and Experimental Validation. Article Snippet: Department of Joint Surgery, Shandong Provincial Hospital, Shandong University, Jinan, Shandong, 250012, China; Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, China; Orthopaedic Research Laboratory, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, 250117, China Article Title: Norwogonin Attenuates Inflammatory Osteolysis and Collagen‐Induced Arthritis via Modulating Redox Signalling and Calcium Oscillations Article Snippet: Cytokines or chemicals used during osteoclast differentiation include macrophage colony‐stimulating factor (M‐CSF, 576406, Biolegend), receptor activator of nuclear factor‐κB ligand (RANKL, 769406, Biolegend), lipopolysaccharide (LPS) (L8274; Sigma‐Aldrich) and Norwogonin (Purity: ≥ 98%) (V34323, Invivochem). .. All primary antibodies employed for western blotting (WB) were derived from rabbit and procured from the following sources: Derivative Assay:Article Title: Norwogonin Attenuates Inflammatory Osteolysis and Collagen‐Induced Arthritis via Modulating Redox Signalling and Calcium Oscillations Article Snippet: Cytokines or chemicals used during osteoclast differentiation include macrophage colony‐stimulating factor (M‐CSF, 576406, Biolegend), receptor activator of nuclear factor‐κB ligand (RANKL, 769406, Biolegend), lipopolysaccharide (LPS) (L8274; Sigma‐Aldrich) and Norwogonin (Purity: ≥ 98%) (V34323, Invivochem). .. All primary antibodies employed for western blotting (WB) were derived from rabbit and procured from the following sources: Article Title: β-Glucan modulates trained immunity to inhibit osteoclast differentiation via NOD2/RIPK2 signaling pathway. Article Snippet: Objective: This study investigated how β-glucan (BG)-induced trained immunity (TI) regulates osteoclast-driven bone resorption, with emphasis on the pivotal signaling axis governing this anti-resorptive memory.. Methods: Bone marrow-derived macrophages (BMMs) were primed with BG for 24 h and subsequently challenged with LPS to establish a TI model, validated by cytokine secretion profiling (IL-1β, IL-6, TNF-α).. Osteoclastogenesis was induced by M-CSF/RANKL and assessed via TRAP staining, F-actin ring formation, immunofluorescence for CTSK/MMP-9, and bone resorption assays. Staining:Article Title: β-Glucan modulates trained immunity to inhibit osteoclast differentiation via NOD2/RIPK2 signaling pathway. Article Snippet: Objective: This study investigated how β-glucan (BG)-induced trained immunity (TI) regulates osteoclast-driven bone resorption, with emphasis on the pivotal signaling axis governing this anti-resorptive memory.. Methods: Bone marrow-derived macrophages (BMMs) were primed with BG for 24 h and subsequently challenged with LPS to establish a TI model, validated by cytokine secretion profiling (IL-1β, IL-6, TNF-α).. Osteoclastogenesis was induced by M-CSF/RANKL and assessed via TRAP staining, F-actin ring formation, immunofluorescence for CTSK/MMP-9, and bone resorption assays. Enzyme-linked Immunosorbent Assay:Article Title: β-Glucan modulates trained immunity to inhibit osteoclast differentiation via NOD2/RIPK2 signaling pathway. Article Snippet: Objective: This study investigated how β-glucan (BG)-induced trained immunity (TI) regulates osteoclast-driven bone resorption, with emphasis on the pivotal signaling axis governing this anti-resorptive memory.. Methods: Bone marrow-derived macrophages (BMMs) were primed with BG for 24 h and subsequently challenged with LPS to establish a TI model, validated by cytokine secretion profiling (IL-1β, IL-6, TNF-α).. Osteoclastogenesis was induced by M-CSF/RANKL and assessed via TRAP staining, F-actin ring formation, immunofluorescence for CTSK/MMP-9, and bone resorption assays. CCK-8 Assay:Article Title: β-Glucan modulates trained immunity to inhibit osteoclast differentiation via NOD2/RIPK2 signaling pathway. Article Snippet: Objective: This study investigated how β-glucan (BG)-induced trained immunity (TI) regulates osteoclast-driven bone resorption, with emphasis on the pivotal signaling axis governing this anti-resorptive memory.. Methods: Bone marrow-derived macrophages (BMMs) were primed with BG for 24 h and subsequently challenged with LPS to establish a TI model, validated by cytokine secretion profiling (IL-1β, IL-6, TNF-α).. Osteoclastogenesis was induced by M-CSF/RANKL and assessed via TRAP staining, F-actin ring formation, immunofluorescence for CTSK/MMP-9, and bone resorption assays. |

