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Proteintech anti ctsk
Anti Ctsk, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ctsk/Cathepsin+K+Antibody/pmc13049295-26-3-5
Average 95 stars, based on 144 article reviews
anti ctsk - by Bioz Stars, 2026-09
95/100 stars

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Related Articles

Immunohistochemistry:

Article Title: EBV Promotes Alveolar Trabecula Resorption via Extracellular Vesicle Remodeling by Group IIA Secreted Phospholipase A2.
Article Snippet: .. Immunohistochemistry and in situ hybridization Immunohistochemistry and EBER in situ hybridization were performed using a Leica Bond Max autostainer following the manufacturer’s instructions (Leica K.K., Tokyo, Japan) AntiCD20 (CD20-L26-L-CE, Novocastra, Wetzlar, Germany), anti-CTSK (11239_1_AP, Proteintech/NBP1_79878, Novus, Centennial, CO), and anti-RANKL antibodies (bs_07747R, Bioss, Woburn, MA), and in situ hybridization using a probe against EBER (Bond ISH EBER; Probe, #PB0589) were all used. ..

In Situ Hybridization:

Article Title: EBV Promotes Alveolar Trabecula Resorption via Extracellular Vesicle Remodeling by Group IIA Secreted Phospholipase A2.
Article Snippet: .. Immunohistochemistry and in situ hybridization Immunohistochemistry and EBER in situ hybridization were performed using a Leica Bond Max autostainer following the manufacturer’s instructions (Leica K.K., Tokyo, Japan) AntiCD20 (CD20-L26-L-CE, Novocastra, Wetzlar, Germany), anti-CTSK (11239_1_AP, Proteintech/NBP1_79878, Novus, Centennial, CO), and anti-RANKL antibodies (bs_07747R, Bioss, Woburn, MA), and in situ hybridization using a probe against EBER (Bond ISH EBER; Probe, #PB0589) were all used. ..

Article Title: EBV promotes alveolar trabecula resorption via extracellular vesicle remodeling by group IIA secreted phospholipase A 2
Article Snippet: Immunohistochemistry and EBER in situ hybridization were performed using a Leica Bond Max autostainer following the manufacturer’s instructions (Leica K.K.) .. Anti-CD20 (CD20-L26-L-CE, Novocastra), anti-CTSK (11,239_1_AP, Proteintech/NBP1_79878, Novus), and anti-RANKL antibodies (bs_07747R, Bioss), and in situ hybridization using a probe against EBER (Bond ISH EBER; Probe, #PB0589) were all used. ..

Membrane:

Article Title: Single-cell analysis links DCUN1D5 to immune remodeling and cisplatin resistance in recurrent osteosarcoma.
Article Snippet: The proteins are separated via 10% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Sigma-Aldrich, USA). .. The membrane is incubated with primary antibodies: anti-DCUN1D5 (1:1000, proteintech), anti-MMP9 (1:500, proteintech), anti-CTSK (1:500, proteintech), anti-AKT (1:1000; Cell Signaling Technology, USA), anti-PI3K (1:1000; Cell Signaling Technology), anti-GSK3β (1:1000; Cell Signaling Technology), anti-p-Akt (Ser-473; 1:1000; Cell Signaling Technology), anti-p-PI3K (Tyr458; 1:1000; Cell Signaling Technology), anti-p- GSK3β (Ser-9; 1:1000; Cell Signaling Technology), anti-p-PI3K (Tyr458; 1:1000; Cell Signaling Technology) and antiGAPDH (1:1000, abcam), followed by secondary antibodies (1:5000, Cell Signaling Technology). .. Post-washing, the signals are detected using a chemiluminescence system (Bio-Rad, USA) and analyzed via the Image Lab software (Bio-Rad).

Article Title: Single-cell analysis links DCUN1D5 to immune remodeling and cisplatin resistance in recurrent osteosarcoma
Article Snippet: The proteins are separated via 10% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Sigma-Aldrich, USA). .. The membrane is incubated with primary antibodies: anti- DCUN1D5 (1:1000, proteintech), anti-MMP9 (1:500, proteintech), anti-CTSK (1:500, proteintech), anti-AKT (1:1000; Cell Signaling Technology, USA), anti-PI3K (1:1000; Cell Signaling Technology), anti-GSK3β (1:1000; Cell Signaling Technology), anti-p-Akt (Ser-473; 1:1000; Cell Signaling Technology), anti-p-PI3K (Tyr458; 1:1000; Cell Signaling Technology), anti-p- GSK3β (Ser-9; 1:1000; Cell Signaling Technology), anti-p-PI3K (Tyr458; 1:1000; Cell Signaling Technology) and anti-GAPDH (1:1000, abcam), followed by secondary antibodies (1:5000, Cell Signaling Technology). .. Post-washing, the signals are detected using a chemiluminescence system (Bio-Rad, USA) and analyzed via the Image Lab software (Bio-Rad).

Incubation:

Article Title: Single-cell analysis links DCUN1D5 to immune remodeling and cisplatin resistance in recurrent osteosarcoma.
Article Snippet: The proteins are separated via 10% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Sigma-Aldrich, USA). .. The membrane is incubated with primary antibodies: anti-DCUN1D5 (1:1000, proteintech), anti-MMP9 (1:500, proteintech), anti-CTSK (1:500, proteintech), anti-AKT (1:1000; Cell Signaling Technology, USA), anti-PI3K (1:1000; Cell Signaling Technology), anti-GSK3β (1:1000; Cell Signaling Technology), anti-p-Akt (Ser-473; 1:1000; Cell Signaling Technology), anti-p-PI3K (Tyr458; 1:1000; Cell Signaling Technology), anti-p- GSK3β (Ser-9; 1:1000; Cell Signaling Technology), anti-p-PI3K (Tyr458; 1:1000; Cell Signaling Technology) and antiGAPDH (1:1000, abcam), followed by secondary antibodies (1:5000, Cell Signaling Technology). .. Post-washing, the signals are detected using a chemiluminescence system (Bio-Rad, USA) and analyzed via the Image Lab software (Bio-Rad).

Article Title: Single-cell analysis links DCUN1D5 to immune remodeling and cisplatin resistance in recurrent osteosarcoma
Article Snippet: The proteins are separated via 10% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Sigma-Aldrich, USA). .. The membrane is incubated with primary antibodies: anti- DCUN1D5 (1:1000, proteintech), anti-MMP9 (1:500, proteintech), anti-CTSK (1:500, proteintech), anti-AKT (1:1000; Cell Signaling Technology, USA), anti-PI3K (1:1000; Cell Signaling Technology), anti-GSK3β (1:1000; Cell Signaling Technology), anti-p-Akt (Ser-473; 1:1000; Cell Signaling Technology), anti-p-PI3K (Tyr458; 1:1000; Cell Signaling Technology), anti-p- GSK3β (Ser-9; 1:1000; Cell Signaling Technology), anti-p-PI3K (Tyr458; 1:1000; Cell Signaling Technology) and anti-GAPDH (1:1000, abcam), followed by secondary antibodies (1:5000, Cell Signaling Technology). .. Post-washing, the signals are detected using a chemiluminescence system (Bio-Rad, USA) and analyzed via the Image Lab software (Bio-Rad).

Article Title: Immune-Derived THBS1-CD47 Axis Induces Cellular Senescence and Suppresses Osteogenesis in Diabetic Periosteum
Article Snippet: Subsequently, tissues were blocked by serum (Invitrogen) for 30 minutes at room temperature. .. Primary antibodies, including anti-CTSK (1:100; Proteintech, 11239-1-AP), anti-Ki67 (1:100; CST, #9129), anti-COL1A1 (1:50; Beyotime, AF6524), anti-F4/80 (1:100; Abcam, ab300421), and THBS1 antibody (1:100; A6.1, Thermo Fisher), were applied and incubated overnight at 4°C. .. After washing, sections were incubated with Alexa Fluor 488 (1:500; Thermo Fisher, ab150113) or Alexa Fluor 647 (1:500; Abcam, A21245), for 1 hour at room temperature.

Western Blot:

Article Title: Spinosin Suppresses RANKL-induced Osteoclastogenesis and Alleviates LPS-induced Cranial Osteolysis: A Study Based on Network Pharmacology and Experimental Validation.
Article Snippet: Department of Joint Surgery, Shandong Provincial Hospital, Shandong University, Jinan, Shandong, 250012, China; Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, China; Orthopaedic Research Laboratory, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, 250117, China

Article Title: Norwogonin Attenuates Inflammatory Osteolysis and Collagen‐Induced Arthritis via Modulating Redox Signalling and Calcium Oscillations
Article Snippet: Cytokines or chemicals used during osteoclast differentiation include macrophage colony‐stimulating factor (M‐CSF, 576406, Biolegend), receptor activator of nuclear factor‐κB ligand (RANKL, 769406, Biolegend), lipopolysaccharide (LPS) (L8274; Sigma‐Aldrich) and Norwogonin (Purity: ≥ 98%) (V34323, Invivochem). .. All primary antibodies employed for western blotting (WB) were derived from rabbit and procured from the following sources: anti‐CTSK (11239‐1‐AP) and anti‐Keap1 (10503‐2‐AP) were obtained from Proteintech (China); anti‐Nrf2 (A0674) and anti‐Nfatc1 (A1539) were obtained from ABclonal (China); ERp57 (2881S) was obtained from Cell Signaling Technology (USA). .. The secondary antibody used in this study was goat anti‐rabbit (SA00001‐2) procured from Proteintech (China).

Derivative Assay:

Article Title: Norwogonin Attenuates Inflammatory Osteolysis and Collagen‐Induced Arthritis via Modulating Redox Signalling and Calcium Oscillations
Article Snippet: Cytokines or chemicals used during osteoclast differentiation include macrophage colony‐stimulating factor (M‐CSF, 576406, Biolegend), receptor activator of nuclear factor‐κB ligand (RANKL, 769406, Biolegend), lipopolysaccharide (LPS) (L8274; Sigma‐Aldrich) and Norwogonin (Purity: ≥ 98%) (V34323, Invivochem). .. All primary antibodies employed for western blotting (WB) were derived from rabbit and procured from the following sources: anti‐CTSK (11239‐1‐AP) and anti‐Keap1 (10503‐2‐AP) were obtained from Proteintech (China); anti‐Nrf2 (A0674) and anti‐Nfatc1 (A1539) were obtained from ABclonal (China); ERp57 (2881S) was obtained from Cell Signaling Technology (USA). .. The secondary antibody used in this study was goat anti‐rabbit (SA00001‐2) procured from Proteintech (China).

Article Title: β-Glucan modulates trained immunity to inhibit osteoclast differentiation via NOD2/RIPK2 signaling pathway.
Article Snippet: Objective: This study investigated how β-glucan (BG)-induced trained immunity (TI) regulates osteoclast-driven bone resorption, with emphasis on the pivotal signaling axis governing this anti-resorptive memory.. Methods: Bone marrow-derived macrophages (BMMs) were primed with BG for 24 h and subsequently challenged with LPS to establish a TI model, validated by cytokine secretion profiling (IL-1β, IL-6, TNF-α).. Osteoclastogenesis was induced by M-CSF/RANKL and assessed via TRAP staining, F-actin ring formation, immunofluorescence for CTSK/MMP-9, and bone resorption assays.

Staining:

Article Title: β-Glucan modulates trained immunity to inhibit osteoclast differentiation via NOD2/RIPK2 signaling pathway.
Article Snippet: Objective: This study investigated how β-glucan (BG)-induced trained immunity (TI) regulates osteoclast-driven bone resorption, with emphasis on the pivotal signaling axis governing this anti-resorptive memory.. Methods: Bone marrow-derived macrophages (BMMs) were primed with BG for 24 h and subsequently challenged with LPS to establish a TI model, validated by cytokine secretion profiling (IL-1β, IL-6, TNF-α).. Osteoclastogenesis was induced by M-CSF/RANKL and assessed via TRAP staining, F-actin ring formation, immunofluorescence for CTSK/MMP-9, and bone resorption assays.

Enzyme-linked Immunosorbent Assay:

Article Title: β-Glucan modulates trained immunity to inhibit osteoclast differentiation via NOD2/RIPK2 signaling pathway.
Article Snippet: Objective: This study investigated how β-glucan (BG)-induced trained immunity (TI) regulates osteoclast-driven bone resorption, with emphasis on the pivotal signaling axis governing this anti-resorptive memory.. Methods: Bone marrow-derived macrophages (BMMs) were primed with BG for 24 h and subsequently challenged with LPS to establish a TI model, validated by cytokine secretion profiling (IL-1β, IL-6, TNF-α).. Osteoclastogenesis was induced by M-CSF/RANKL and assessed via TRAP staining, F-actin ring formation, immunofluorescence for CTSK/MMP-9, and bone resorption assays.

CCK-8 Assay:

Article Title: β-Glucan modulates trained immunity to inhibit osteoclast differentiation via NOD2/RIPK2 signaling pathway.
Article Snippet: Objective: This study investigated how β-glucan (BG)-induced trained immunity (TI) regulates osteoclast-driven bone resorption, with emphasis on the pivotal signaling axis governing this anti-resorptive memory.. Methods: Bone marrow-derived macrophages (BMMs) were primed with BG for 24 h and subsequently challenged with LPS to establish a TI model, validated by cytokine secretion profiling (IL-1β, IL-6, TNF-α).. Osteoclastogenesis was induced by M-CSF/RANKL and assessed via TRAP staining, F-actin ring formation, immunofluorescence for CTSK/MMP-9, and bone resorption assays.



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Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression <t>of</t> <t>Nfatc1,</t> C-fos, Atp6v0d2, <t>Ctsk,</t> Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).
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Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression <t>of</t> <t>Nfatc1,</t> C-fos, Atp6v0d2, <t>Ctsk,</t> Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).
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Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression <t>of</t> <t>Nfatc1,</t> C-fos, Atp6v0d2, <t>Ctsk,</t> Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).
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Chemokine (C-X-C motif) ligand 5 (CXCL5) inhibits receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclast differentiation. a), c), and e) Immunofluorescence staining confirmed that CXCL5 (1 or 50 ng/ml) inhibits RANKL-induced differentiation of RAW264.7 cells into cathepsin K <t>(CTSK)-positive</t> osteoclast-like cells. b), d), and f) CXCL5 decreased the differentiation of RAW264.7 cells into tartrate-resistant acid <t>phosphatase</t> <t>(TRAP)-positive</t> osteoclast-like cells. Purple indicates TRAP expression, and cyan indicates nuclei. g) and h) Western blot analysis showed that CXCL5 (1 ng/ml) inhibited RANKL-induced differentiation of RAW264.7 cells by suppressing the transcription factor nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), resulting in decreased CTSK expression at 72 hours of treatment. Data are presented as the mean (standard error of the mean (SEM)) and analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001. DAPI, 4′,6-diamidino-2-phenylindole.
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Image Search Results


Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression of Nfatc1, C-fos, Atp6v0d2, Ctsk, Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).

Journal: Materials Today Bio

Article Title: An alkaline nanosized platelet vesicle-based hydrogel for the treatment of osteoporotic bone defects

doi: 10.1016/j.mtbio.2026.103000

Figure Lengend Snippet: Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression of Nfatc1, C-fos, Atp6v0d2, Ctsk, Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).

Article Snippet: Western blotting was used to evaluate the protein expression of NFATc1 (sc-7294, Santa Cruz), c-Fos (sc-166940, Santa Cruz), CTSK (11239-1-AP, ProteinTech), and β-actin (20536-1-AP, ProteinTech).

Techniques: In Vitro, Staining, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Electron Microscopy, Western Blot

Chemokine (C-X-C motif) ligand 5 (CXCL5) inhibits receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclast differentiation. a), c), and e) Immunofluorescence staining confirmed that CXCL5 (1 or 50 ng/ml) inhibits RANKL-induced differentiation of RAW264.7 cells into cathepsin K (CTSK)-positive osteoclast-like cells. b), d), and f) CXCL5 decreased the differentiation of RAW264.7 cells into tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells. Purple indicates TRAP expression, and cyan indicates nuclei. g) and h) Western blot analysis showed that CXCL5 (1 ng/ml) inhibited RANKL-induced differentiation of RAW264.7 cells by suppressing the transcription factor nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), resulting in decreased CTSK expression at 72 hours of treatment. Data are presented as the mean (standard error of the mean (SEM)) and analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001. DAPI, 4′,6-diamidino-2-phenylindole.

Journal: Bone & Joint Research

Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection

doi: 10.1302/2046-3758.153.BJR-2025-0290.R1

Figure Lengend Snippet: Chemokine (C-X-C motif) ligand 5 (CXCL5) inhibits receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclast differentiation. a), c), and e) Immunofluorescence staining confirmed that CXCL5 (1 or 50 ng/ml) inhibits RANKL-induced differentiation of RAW264.7 cells into cathepsin K (CTSK)-positive osteoclast-like cells. b), d), and f) CXCL5 decreased the differentiation of RAW264.7 cells into tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells. Purple indicates TRAP expression, and cyan indicates nuclei. g) and h) Western blot analysis showed that CXCL5 (1 ng/ml) inhibited RANKL-induced differentiation of RAW264.7 cells by suppressing the transcription factor nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), resulting in decreased CTSK expression at 72 hours of treatment. Data are presented as the mean (standard error of the mean (SEM)) and analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001. DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho-phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotechnology, USA).

Techniques: Immunofluorescence, Staining, Expressing, Western Blot, Comparison